Date of Award
Spring 2022
Document Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
Committee Chairperson
Sean Buskirk, Ph.D.
Committee Member
John Pisciotta, Ph.D.
Committee Member
Jessica Sowa, Ph.D.
Abstract
With the ongoing COVID-19 pandemic, the use of alcohol-based antiseptics by the general public has greatly increased. While this form of antimicrobial is effective in stopping the spread of both bacterial and viral infections, little research has been conducted in the development of alcohol tolerance by microorganisms or its mechanisms. In this study, experimental evolution was conducted on four separate strains of Staphylococcus aureus to determine if tolerance can develop towards moderate to high concentrations of ethanol. Once ethanol tolerance was established in the evolved populations, whole genome sequencing was performed to identify the potential mutations responsible for the observed alcohol tolerance.
Recommended Citation
Perveiler, Vaughn, "Investigating the development of alcohol tolerance by the opportunistic pathogen Staphylococcus aureus" (2022). West Chester University Master’s Theses. 257.
https://digitalcommons.wcupa.edu/all_theses/257